Part:BBa_I723028:Design

PCA biosynthesis operon

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 927
  Illegal EcoRI site found at 3549
  Illegal PstI site found at 1070
  Illegal PstI site found at 4554
  Illegal PstI site found at 5103
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 927
  Illegal EcoRI site found at 3549
  Illegal PstI site found at 1070
  Illegal PstI site found at 4554
  Illegal PstI site found at 5103
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 927
  Illegal EcoRI site found at 3549
  Illegal BamHI site found at 276
  Illegal BamHI site found at 1482
  Illegal XhoI site found at 220
  Illegal XhoI site found at 379
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 927
  Illegal EcoRI site found at 3549
  Illegal PstI site found at 1070
  Illegal PstI site found at 4554
  Illegal PstI site found at 5103
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 927
  Illegal EcoRI site found at 3549
  Illegal PstI site found at 1070
  Illegal PstI site found at 4554
  Illegal PstI site found at 5103
  Illegal NgoMIV site found at 142
  Illegal NgoMIV site found at 1151
  Illegal NgoMIV site found at 1298
  Illegal NgoMIV site found at 1316
  Illegal NgoMIV site found at 1320
  Illegal NgoMIV site found at 2124
  Illegal NgoMIV site found at 2298
  Illegal AgeI site found at 5392
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 5535
  Illegal BsaI.rc site found at 2234
  Illegal BsaI.rc site found at 4611
  Illegal BsaI.rc site found at 5017
  Illegal BsaI.rc site found at 5044
  Illegal SapI.rc site found at 4031
  

Design Notes

The entire operon was too large to clone at once; we designed our experiments to do it in two fragments.

Source

Cloned from Pseudomonas aeruginosa PA01 genomic sequence.